陆卫

      博士，博士后

      学习工作经历

      2009-2013，四川农业大学动物医学，农学学士

      2013-2021，西南大学遗传学，理学博士

      2022至今， 西南大学生物学，博士后

      研究领域

      1. 基因组编辑

      2. 丝蛋白基因改造

      成果情况

      代表性论文/论著

      1. Chang, J.; Chen, X.; Wang, R.; Shi, R.; Wang, X.; Lu, W.; Ma, S.; Xia, Q. High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression. Molecules 2020, 25, doi:10.3390/molecules25020353.

      2. Hu, W.; Lu, W.; Wei, L.; Zhang, Y.; Xia, Q. Molecular nature of dominant naked pupa mutation reveals novel insights into silk production in Bombyx mori. Insect Biochem. Mol. Biol. 2019, 109, 52–62, doi:10.1016/j.ibmb.2019.04.006.

      3. Li, Y.; Ma, S.; Le Sun; Zhang, T.; Chang, J.; Lu, W.; Chen, X.; Liu, Y.; Wang, X.; Shi, R.; et al. Programmable Single and Multiplex Base-Editing in Bombyx mori Using RNA-Guided Cytidine Deaminases. G3 (Bethesda) 2018, 8, 1701–1709, doi:10.1534/g3.118.200134.

      4. Liu, Y.; Ma, S.; Wang, X.; Chang, J.; Gao, J.; Shi, R.; Zhang, J.; Lu, W.; Liu, Y.; Zhao, P.; et al. Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9. Insect Biochem. Mol. Biol. 2014, 49, 35–42, doi:10.1016/j.ibmb.2014.03.010.

      5. Liu, Y.; Ma, S.; Chang, J.; Zhang, T.; Wang, X.; Shi, R.; Zhang, J.; Lu, W.; Liu, Y.; Xia, Q. Tissue-specific genome editing of laminA/C in the posterior silk glands of Bombyx mori. J. Genet. Genomics 2017, 44, 451–459, doi:10.1016/j.jgg.2017.09.003.

      6. Liu, Y.; Liang, Y.; Yang, C.; Shi, R.; Lu, W.; Wang, X.; Wang, R.; Xia, Q.; Ma, S. A deep insight into the transcriptome of midgut and fat body reveals the toxic mechanism of fluoride exposure in silkworm. Chemosphere 2021, 262, 127891, doi:10.1016/j.chemosphere.2020.127891.

      7. Lu, W.; Wang, R.; Wang, P.; Ma, S.; Xia, Q. Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines. Insects 2021, 12, doi:10.3390/insects12121081.

      8. Lu, W.; Lan, X.; Zhang, T.; Sun, H.; Ma, S.; Xia, Q. Precise Characterization of Bombyx mori Fibroin Heavy Chain Gene Using Cpf1-Based Enrichment and Oxford Nanopore Technologies. Insects 2021, 12, doi:10.3390/insects12090832.

      9. Ma, S.; Chang, J.; Wang, X.; Liu, Y.; Zhang, J.; Lu, W.; Gao, J.; Shi, R.; Zhao, P.; Xia, Q. CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori. Sci Rep 2014, 4, 4489, doi:10.1038/srep04489.

      10. Ma, S.; Shi, R.; Wang, X.; Liu, Y.; Chang, J.; Gao, J.; Lu, W.; Zhang, J.; Zhao, P.; Xia, Q. Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor. Scientific Reports 2014, 4, doi:10.1038/srep06867.

      11. Ma, S.; Liu, Y.; Liu, Y.; Chang, J.; Zhang, T.; Wang, X.; Shi, R.; Lu, W.; Xia, X.; Zhao, P.; et al. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites. Insect Biochem. Mol. Biol. 2017, 83, 13–20, doi:10.1016/j.ibmb.2017.02.003.

      12. Ma, S.; Zhang, J.; Lu, W.; Liu, Y.; Xia, Q. SAA-Cas9: A tunable genome editing system with increased bio-safety and reduced off-target effects. J. Genet. Genomics 2019, 46, 145–148, doi:10.1016/j.jgg.2019.02.004.

      13. Ma, S.-Y.; Liu, Y.-Y.; Lu, W.; Wang, X.-G.; Le Sun; Yu, K.; Xia, Q.-Y. Targeted activation of BmCyclinE in Bombyx mori using designer TAL effectors. Insect Sci. 2019, 26, 1055–1058, doi:10.1111/1744-7917.12613.

      14. Wang, X.; Ma, S.; Liu, Y.; Lu, W.; Le Sun; Zhao, P.; Xia, Q. Transcriptional repression of endogenous genes in BmE cells using CRISPRi system. Insect Biochem. Mol. Biol. 2019, 111, 103172, doi:10.1016/j.ibmb.2019.05.007.

      15. Yan, H.; Liu, Q.; Wen, F.; Bai, B.; Wen, Y.; Chen, W.; Lu, W.; Lin, Y.; Xia, Q.; Wang, G. Characterization and potential application of an α-amylase (BmAmy1) selected during silkworm domestication. Int. J. Biol. Macromol. 2021, 167, 1102–1112, doi:10.1016/j.ijbiomac.2020.11.064.

    联系方式

     Email：luw@office365.swu.edu.cn