施 润

     博士、博士后

     学习工作经历

     2020.07--至今，西南大学，博士后

     2012.09--2020.06，西南大学，生物化学与分子生物学专业，硕博连读，理学博士

     2008.09--2012.06，河南科技大学，动物科学专业，农学学士

     研究领域

1. 家蚕全基因组编辑及应用

2. 家蚕丝腺的遗传改良

     成果情况

   代表性论文/论著

  1. Shi, R.; Ma, S.; He, T.; Peng, J.; Zhang, T.; Chen, X.; Wang, X.; Chang, J.; Xia, Q.; Zhao, P. Deep Insight into the Transcriptome of the Single Silk Gland of Bombyx mori[J]. Int. J. Mol. Sci. 2019, 20(10):2491.

     2. Ma S.*, Shi R.*, Wang X., et al. Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor[J]. Scientific Reports. 2014, 4:6867.

     3. Ma S, Chang J, Wang X, Liu Y, Zhang J, Lu W, Gao J, Shi R, Zhao P, Xia Q. CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori. Sci Rep. 2014 Mar 27;4:4489.

     4. Liu Y, Ma S, Wang X, Chang J, Gao J, Shi R, Zhang J, Lu W, Liu Y, Zhao P, Xia Q. Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9. Insect Biochem Mol Biol. 2014 Jun;49:35-42.

     5. Ma S, Liu Y, Liu Y, Chang J, Zhang T, Wang X, Shi R, Lu W, Xia X, Zhao P, Xia Q. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites. Insect Biochem Mol Biol. 2017 Apr; 83:13-20.

     6. Ma S, Xia X, Li Y, Sun L, Liu Y, Liu Y, Wang X, Shi R, Chang J, Zhao P, Xia Q. Increasing the yield of middle silk gland expression system through transgenic knock-down of endogenous sericin-1. Mol Genet Genomics. 2017 Aug;292(4):823-831.

     7. Liu Y, Ma S, Chang J, Zhang T, Wang X, Shi R, Zhang J, Lu W, Liu Y, Xia Q. Tissue-specific genome editing of laminA/C in the posterior silk glands of Bombyx mori. J Genet Genomics. 2017 Sep 20;44(9):451-459.

     8. Li Y, Ma S, Sun L, Zhang T, Chang J, Lu W, Chen X, Liu Y, Wang X, Shi R, Zhao P, Xia Q. Programmable Single and Multiplex Base-Editing in Bombyx mori Using RNA-Guided Cytidine Deaminases. G3 (Bethesda). 2018 May  4;8(5):1701-1709.

     9. Wang XG, Ma SY, Chang JS, Shi R, Wang RL, Zhao P, Xia QY. Programmable activation of Bombyx gene expression using CRISPR/dCas9 fusion systems. Insect Sci. 2019 Dec;26(6):983-990.

     10. Chang J, Chen X, Wang R, Shi R, Wang X, Lu W, Ma S, Xia Q. High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression. Molecules. 2020 Jan 15;25(2):353.

     11. Chang J, Wang R, Yu K, Zhang T, Chen X, Liu Y, Shi R, Wang X, Xia Q, Ma S. Genome-wide CRISPR screening reveals genes essential for cell viability and resistance to abiotic and biotic stresses in Bombyx mori. Genome Res. 2020 May;30(5):757-767.

     12. Liu Y, Liang Y, Yang C, Shi R, Lu W, Wang X, Wang R, Xia Q, Ma S. A deep insight into the transcriptome of midgut and fat body reveals the toxic mechanism of fluoride exposure in silkworm. Chemosphere. 2020 Aug 6;262:127891.

    联系方式

     Email：runshi@swu.edu.cn