李志清

研究员、研究生导师

重庆市引进高层次人才

学习工作经历

2021.07-至今：    研究员，西南大学前沿交叉学科研究院生物学研究中心

2020.10-2021.06：副研究员，西南大学前沿交叉学科研究院生物学研究中心

2014.10-2020.09：副研究员，西南大学家蚕基因组生物学国家重点实验室

2012.10-2014.09：日本学术振兴会外国人特别研究员, 日本九州大学

2009.10-2012.09：博士，生物学专业，日本九州大学

2007.09-2009.07：硕士，生物化学与分子生物学专业，西南大学

2003.09-2007.07：学士，蚕学专业，西南大学

学术兼职

2020-至今：重庆劳动教育科学研究会会员

2020-至今：Current Genomics，编委（Review Editor）

2021-至今：Frontiers in Genetics，编委（Review Editor）

2021-至今：Frontiers in Insect Science，编委（Review Editor）

研究领域

基础研究：以家蚕为模式生物，主要开展表观遗传调控、蛋白质翻译后修饰、家蚕抗病素材创制、功能性蚕丝蛋白材料开发等研究工作。

应用基础研究：构建基于人工饲料的家蚕智能化养殖技术体系。

科研项目

1.国家自然科学基金青年基金项目，家蚕驯化过程中组蛋白修饰对丝蛋白合成的调控研究，2016.01-2018.12，主持。

2.重庆市自然科学基金面上项目，家蚕翻译控制蛋白 TCTP 的抗病毒机制及高抗性素材创制研究，2023.08-2026.07，主持。

3.重庆市教委科学技术研究计划项目，家蚕DNA甲基化修饰调控丝腺发育的表观遗传机制研究，2024.01-2025.12，主持。

4.重庆市自然科学基金项目，长江上游种质资源保护与创制应用技术预见分析，2021.07-2021.12，主持。

5.国家自然科学基金重点项目，人工饲料饲养家蚕的肠道微生物菌群变化机制与干预研究，2021.01-2025.12，主研。

6.国家自然科学基金重点项目，基于CRISPR/Cas9的家蚕丝蛋白基因全位点饱和编辑与丝纤维遗传改良研究，2016.01-2020.12，主研。

7.重庆市人力资源和社会保障局，家蚕腺嘌呤甲基化修饰介导的丝蛋白高效合成素材研究，2019.01-2020.12，主持。

8.中央高校基本科研业务费重点项目，DNA 6mA和RNA m6A修饰调控家蚕丝蛋白合成研究，2019.01-2021.12，主持。

9.中央高校基本科研业务费一般项目，家蚕驯化过程中丝腺组蛋白修饰的表观遗传变化，2015.01-2017.12，主持。

荣誉获奖

1.2022年获西南大学2020-2022学年度“优秀教师”荣誉称号。

2.2019年西南大学优秀研究生导学团队。

3.2018年重庆市研究生导师团队。

4.2014年入选重庆市引进高层次人才。

发表论文（*为通讯作者）

1.Feng Chen, Hao Guo, Weiqun Lan, Mingyi Zhou, Wenjing Geng, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li*. 2023. Targeted DNA N6-methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori. Insect Science, 1-5.

2.Hao Guo, Feng Chen, Mingyi Zhou, Weiqun Lan, Wenchang Zhang, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li*. 2023. CRISPR-Cas9-Mediated Mutation of Methyltransferase METTL4 Results in Embryonic Defects in Silkworm Bombyx mori. International Journal of Molecular Sciences, 24, 3468.

3.Wenchang Zhang, Zhiqing Li, Weiqun Lan, Hao Guo, Feng Chen, Feng Wang, Guanwang Shen, Qingyou Xia, Ping Zhao*. 2023. Bioengineered Silkworm Model for Expressing Human Neurotrophin-4 with Potential Biomedical Application. Frontiers in Physiology, 13:1104929. (Co-first author)

4.Chenchen Lu, Zhiqing Li*, Wenchang Zhang, Hao Guo, Weiqun Lan, Guanwang Shen, Qingyou Xia, Ping Zhao. 2022. SUMOylation of Translationally Regulated Tumor Protein Modulates Its Immune Function. Frontiers in Immunology, 13:807097.

5.Li Chang, Zhiqing Li*, Hao Guo, Wenchang Zhang, Weiqun Lan, Jue Wang, Guanwang Shen, Qingyou Xia, Ping Zhao. 2021. Function of Polyamines in Regulating Cell Cycle Progression of Cultured Silkworm Cells. Insects, 12(7), 624.

6.Chenchen Lu, Zhiqing Li*, Li Chang, Zhaoming Dong, Pengchao Guo, Qingyou Xia, Ping Zhao. 2020. Efficient Delivery of dsRNA and DNA in Cultured Silkworm Cells for Gene Function Analysis Using PAMAM Dendrimers System. Insects, 11(1), 12.

7.Zhaohui Wang, Yang Li, Bing Hou, Mira I. Pronobis, Mingqiao Wang, Yuemeng Wang, Guangcun Cheng, Weining Weng, Yiqiang Wang, Yanfang Tang, Xuefan Xu, Rong Pan, Fei Lin, Nan Wang, Ziqing Chen, Shiwei Wang, Luyan zulie Ma, Yangrui Li, Dongliang Huang, Li Jiang, Zhiqiang Wang, Wenfang Zeng, Ying Zhang, Xuemei Du, Ying Lin, Zhiqing Li, Qingyou Xia, Jing Geng, Huaping Dai, Yuan Yu, Xiao-dong Zhao, Zheng Yuan, Jian Yan, Qinghua Nie, Xiquan Zhang Kun Wang, Fuling Chen, Qin Zhang, Yuxian Zhu, Susan Zheng, Kenneth D. Poss, Sheng-ce Tao, Xun Meng*. 2020. An Array of 60,000 Antibodies for Proteome-Scale Antibody Generation and Target Discovery. Science Advances, 6(11), eaax2271.

8.Zhiqing Li*, Ping Zhao, Qingyou Xia*. 2019. Epigenetic Methylations on N6-Adenine and N6-Adenosine with the Same Input but Different Output. International Journal of Molecular Sciences, 20(12), 2931.

9.Bingqian Li, Zhiqing Li*, Chenchen Lu, Li Chang, Dongchao Zhao, Guanwang Shen, Takahiro Kusakabe, Qingyou Xia, Ping Zhao. 2019. Heat Shock Cognate 70 Functions as A Chaperone for the Stability of Kinetochore Protein CENP-N in Holocentric Insect Silkworms. International Journal of Molecular Sciences, 20(23), 5823.

10.Bingqian Li, Xiaoyan Wang, Zhiqing Li*, Chenchen Lu, Quan Zhang, Li Chang, Wanshun Li, Tingcai Cheng, Qingyou Xia, Ping Zhao. 2019. Transcriptome-wide analysis of N6-methyladenosine uncovers its regulatory role in gene expression in the lepidopteran Bombyx mori. Insect Molecular Biology, 28(5).703-715.

11.Xiaoyan Wang, Zhiqing Li*, Quan Zhang, Bingqian Li, Chenchen Lu, Wanshun Li, Tingcai Cheng, Qingyou Xia, Ping Zhao. 2018. DNA methylation on N6-adenine in lepidopteran Bombyx mori. BBA-Gene Regulatory Mechanisms, 1861. 815-825.

12.Zhiqing Li, Qixin Cui, Xiaoyan Wang, Bingqian Li, Dongchao Zhao, Qingyou Xia, Ping Zhao*. 2017.  Functions and substrates of NEDDylation during cell cycle in the silkworm, Bombyx mori. Insect Biochemistry and Molecular Biology, 90. 101-112.

13.Zhiqing Li, Qixin Cui, Jian Xu, Daojun Cheng, Xiaoyan Wang, Bingqian Li, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe*, Ping Zhao*. 2017. SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori. Scientific Reports, 7. 15536.

14.Tingcai Cheng, Jiaqi Wu, Yuqian Wu, Rajendra V. Chilukuri, Lihua Huang, Kohji Yamamoto, Li Feng, Wanshun Li, Zhiwei Chen, Huizhen Guo, Jianqiu Liu 1 , Shenglong Li, Xiaoxiao Wang, Li Peng, Duolian Liu, Youbing Guo, Bohua Fu, Zhiqing Li, Chun Liu, Yuhui Chen, Archana Tomar, Frederique Hilliou, Nicolas Montagné, Emmanuelle Jacquin-Joly, Emmanuelle d'Alençon, Rakesh K. Seth, Raj K. Bhatnagar, Akiya Jouraku, Takahiro Shiotsuki, Keiko Kadono-Okuda, Amornrat Promboon, Guy Smagghe, Kallare P. Arunkumar, Hirohisa Kishino , Marian R. Goldsmith, Qili Feng, Qingyou Xia*, Kazuei Mita*. 2017. Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest. Nature Ecology & Evolution, 1. 1747–1756.

15.Zhiqing Li, Hiroaki Mon, Hitoshi Mitsunobu, Li Zhu, Jian Xu, Jae Man Lee, Takahiro Kusakabe*. 2014. Dynamics of Polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage. Insect Biochemistry and Molecular Biology, 55. 9-18.

16.Zhiqing Li, Hiroaki Mon, Jian Xu, Li Zhu, Jae Man Lee, Takahiro Kusakabe*. 2014. A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori. Insect Biochemistry and Molecular Biology, 51. 71-79.

17.Ling Wei, Zhiqing Li, Daojun Cheng, Takahiro Kusakabe, Minhui Pan, Yonghu Wang, Cheng Lu*. 2014. RNAi silencing of SoxE gene suppresses cell proliferation in the silkworm BmN4 cells. Molecular Biology Reports, 41. 4769-4781. (Co-first author)

18.Zhiqing Li, Daojun Cheng, Hiroaki Mon, Li Zhu, Jian Xu, Tsuneyuki Tatsuke, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe*. 2013. Cell cycle-dependent recruitment of Polycomb proteins to the ASNS promoter counteracts C/ebp-mediated transcriptional activation in Bombyx mori. PLoS One, 8. 1, e52320.

19.Zhiqing Li, Li Zhu, Jian Xu, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe*. 2013. Amino acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells. Archives of Insect Biochemistry and Physiology, 83. 2, 57–68.

20.Zhiqing Li, Daojun Cheng, Hiroaki Mon, Tsuneyuki Tatsuke, Li Zhu, Jian Xu, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe*. 2012. Genome-wide identification of Polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori. PLoS One, 7. 4, e34330.

21.Zhiqing Li, Tsuneyuki Tatsuke, Kosuke Sakashita, Li Zhu, Jian Xu, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe*. 2012. Identification and characterization of Polycomb group genes in the silkworm, Bombyx mori. Molecular Biology Reports, 39. 5, 5575-5588.

22.Zhiqing Li, Daojun Cheng, Ling Wei, Ping Zhao, Xu Shu, Lin Tang, Zhonghuai Xiang, Qingyou Xia*. 2010. The silkworm homolog of Methoprene-tolerant (Met) gene reveals sequence conservation but function divergence. Insect Science, 17, 313–324.

联系方式

地址：重庆市北碚区西南大学前沿交叉学科研究院生物学研究中心

Email：lizhiqing@swu.edu.cn