王根洪

研究员、硕士生导师

简历：

1999-2003     西南农业大学 本科生

2003-2008     西南农业大学（西南大学） 博士生（硕博连读）

2008-2012     重庆大学农学及生命科学研究院 讲  师

2012-2023      西南大学副研究员

2014-2016     美国康奈尔大学附属BTI研究所访问学者

2023至今   西南大学研究员

主要科研领域与方向：

1. 基于昆虫-植物互作的动物营养利用调控机制研究

从鳞翅目昆虫-植物二元系统协同进化关系入手，整合基因组学、分子生物学、遗传学和生物化学等研究手段，系统研究调控昆虫进食和营养消化的分子机制，为提升有益昆虫（家蚕）营养利用效率和防控有害昆虫提供思路和分子靶标。比较分析昆虫与哺乳动物营养利用调控机制的异同点，开发调节动物饱腹感及体重增减的生物制剂。

2. 基于基因工程的植物品种素材创制

在解析植物抗虫、抗逆等分子调控机制的基础上，通过CRISPR/Cas9基因编辑、过表达等手段对相应基因表达水平进行调控，在现行植物品种上定向创制更加优良的品种素材。

科学成就：

1. 博士期间，建立了基于转基因RNAi和增量表达抗病毒蛋白的家蚕抗病分子育种技术体系，制作获得了对家蚕核型多角体病毒（BmNPV）具有增强抗性的转基因家蚕素材数个。

2. 作为主研人员，全程参与了家蚕全基因组基因芯片的研制。参加了西南大学主持的家蚕基因组计划及“十一五”、“十二五”家蚕“973”、“863”、“948”及国家自然科学基金重点项目。

3. 主持国家重点研发计划子课题、国家自然科学基金、重庆市自然科学基金、重庆市基础科学与前沿技术研究项目、中央高校科研业务费等各类科研项目20余项。近年来已在国内外高水平期刊发表论文30余篇，参与编写专著2部；获得专利授权10余项，其中第一专利权人6 项，申请专利2项。任教育部学位论文评审专家及全国研究生教育评估监测专家。

代表性论文/论著：

1. Wen Feng, Wang Jing, Shang Deli, Yan Hao, Yuan Xingli, Wang Yuanqiang, Xia Qingyou, Genhong Wang#(Corresponding author) (2023). Non-classical digestive lipase BmTGL selected by gene amplification reduces the effects of mulberry inhibitor during silkworm domestication. International Journal of Biological Macromolecules, 229 (2023) 589–599.

2. Liu Lijing, Zhao Dongchao, Wang Genhong, He Qingxiu, Song Yuwei, Jiang Yulu, Xia Qingyou and Zhao Ping (2023). Adaptive Changes in Detoxification Metabolism and Transmembrane Transport of Bombyx mori Malpighian Tubules to Artificial Diet. Int. J. Mol. Sci., 2023, 24, 9949.

3. Chen,Wenwen, Wang Genhong, Yi Meiqin, Gao Tianke, Song Chunman, Gao Qian, Xia Qingyou, Xiang Haiying (2023). Whole‑genome identification and salt‑ and ABA‑induced expression trends of the Nicotiana tabacum CKX gene family. Plant Biotechnology Reports, 17:389–399.

4. Yan Hao，Wen Feng，Xiang Haiying，Wen Yuchan，Shang Deli，Liu Anyang， Niu Yicheng，Xia Qingyou, Wang Genhong#(Corresponding author) (2022). Biochemical characterization and overexpression of an α-amylase (BmAmy) in silkworm, Bombyx mori. Insect Molecular Biology, 31:251–259.

5. Li Zhaowu, Gao Junping, Wang Genhong, Wang Shuaibin, Chen Kai, Pu Wenxuan, Wang Yaofu, Xia Qingyou＃ and Fan Xiaorong＃(2022). Genome-Wide Identification and Characterization of GASA Gene Family in Nicotiana tabacum. Frontiers in Genetics, 12 , Article 768942

6. Yan Hao, Liu qingsong, Wen feng, Bai bingchuan, Wen Yuchan, Chen Wenwen, Lu Wei, Lin Ying, Xia Qingyou, Wang Genhong #(Corresponding author) (2021). Characterization and potential application of an α-amylase (BmAmy1) selected during silkworm domestication. International Journal of Biological Macromolecules, 167 (2021) 1102–1112.

7. Li Yueyue, Zhao min, Chen Wenwen, Du Hongyi, Xie Xiaodong, Wang Daibin, Dai Ya, Xia Qingyou, Wang Genhong# (Corresponding author) (2020). Comparative transcriptomic analysis reveals that multiple hormone signal transduction and carbohydrate metabolic pathways are affected by Bacillus cereus in Nicotiana tabacum. Genomics, 112, 4254–4267.

8. DaoYuan Qin, Genhong Wang, ZhaoMing Dong, QingYou Xia, Ping Zhao (2020). Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet. Insects, 30;11(12):851.

9. Chen Yanfei, Bai Bingchuan, Yan Hao, Wen Feng, Qin Daoyuan, Jander Georg, Xia Qingyou, Wang Genhong#(Corresponding author) (2019). Systemic disruption of the homeostasis of transfer RNA isopentenyltransferase causes growth and development abnormalities in Bombyx mori. Insect molecular biology, 28(3), 380–391.

  10. Li Yueyue, Bai Bingchuan, Wen Feng, Zhao Min, Xia Qingyou, Yang Da-Hai*, and Genhong Wang*(Corresponding author) (2019). Genome-Wide Identification and Expression Analysis of HD-ZIP I Gene Subfamily in Nicotiana tabacum. Genes, 10(8): 575.

11.Wang Genhong, Chen Yanfei, Zhang Xiaoying, Bai Bingchuan, Yan Hao, Qin Daoyuan, Xia Qingyou (2018). Selection of reference genes for tissue/organ samples on day 3 fifth-instar larvae in silkworm, Bombyx mori. Arch Insect Biochem Physiol 98(2), e21458.

12. Wang Genhong, Wang Pei, Gao Yulong, Li Yueyue, Wu Lei, Gao Junping, Zhao Min, Xia Qingyou (2018). Isolation and functional characterization of a novel FLOWERING LOCUS T homolog (NtFT5) in Nicotiana tabacum. Journal of plant physiology. 2018;231:393-401.

13.Wu Lei, Zhang Xiaoying, Xu Bingxin, Li Yueyue, Jia Ling, Wang Rengang, Ren Xueliang, Wang Genhong#(Corresponding author), Xia Qingyou (2018). Identification and expression analysis of EDR1-like genes in tobacco (Nicotiana tabacum) in response to Golovinomyces orontii. PeerJ 6, e5244.

14. Xingtan Zhang, Genhong Wang, Junping Gao, Mengyu Nie, Wenshan Liu and Qingyou Xia (2016) Functional analysis of NtMPK2 uncovers its positive role in response to Pseudomonas syringae pv. tomato DC3000 in tobacco. Plant Mol Biol, 90, 19-31.

15. Junping Gao, Genhong Wang＃ (Co-first author), Sanyuan Ma, Xiaodong Xie, Xiangwei Wu, Xingtan Zhang, Yuqian Wu, Ping Zhao and Qingyou Xia (2015). CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum. Plant Mol Biol, 87, 99-110.

16. Xiaodong Xie, Genhong Wang＃ (Co-first author), Ling Yang, Tingcai Cheng, Junping Gao, Yuqian Wu and Qingyou Xia (2015). Cloning and characterization of a novel Nicotiana tabacum ABC transporter involved in shoot branching. Physiol Plant, 153, 299-306.

17. Xingtan Zhang, Mengyun Nie, Qian Zhao, Yuqian Wu, Genhong Wang and Qingyou Xia (2015) Genome-wide patterns of genetic variation among silkworms. Mol Genet Genomics, 290, 1575-87.

18. Genhong Wang, Liang Jiang, Li Zhu, Ting-Cai Cheng, Wei-Huan Niu, Ya-Fei Yan and Qing-You Xia.(2013). “Characterization of Argonaute family members in the silkworm, Bombyx mori.” Insect Science. 00:1-14.

19. Jiang, L., P. Zhao, T. Cheng, Q. Sun, Z. Peng, Y. Dang, X. Wu, G. Wang, S. Jin, P. Lin & Q. Xia (2013) A transgenic animal with antiviral properties that might inhibit multiple stages of infection. Antiviral Res, 98, 171-3.

20. Xiaodong Xie, Tingcai Cheng, Genhong Wang, Jun Duan, Weihuan Niu and Qingyou Xia (2012) Genome-wide analysis of the ATP-binding cassette (ABC) transporter gene family in the silkworm, Bombyx mori. Mol Biol Rep, 39, 7281-91.

21. Jiang, L., G. Wang, T. Cheng, Q. Yang, S. Jin, G. Lu, F. Wu, Y. Xiao, H. Xu and Q. Xia (2012). "Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms." Arch Virol.

22. Zhao, P., Z. Dong, J. Duan, G. Wang, L. Wang, Y. Li, Z. Xiang and Q. Xia (2012). "Genome-wide identification and immune response analysis of serine protease inhibitor genes in the silkworm, Bombyx mori." PLoS One 7(2): e31168.

23. Jiang, L., T. Cheng, P. Zhao, Q. Yang, G. Wang, S. Jin, P. Lin, Y. Xiao & Q. Xia (2012) Resistance to BmNPV via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, Bombyx mori. PLoS One, 7, e41838.

24. Jiang, L., P. Zhao, G. Wang, T. Cheng, Q. Yang, S. Jin, P. Lin, Y. Xiao, Q. Sun & Q. Xia (2012) Comparison of factors that may affect the inhibitory efficacy of transgenic RNAi targeting of baculoviral genes in silkworm, Bombyx mori. Antiviral Res, 97, 255-263.

25. Xingtan Zhang, Tingcai Cheng, Genhong Wang, Yafei Yan, Qingyou Xia(2012). Cloning and evolutionary analysis of tobacco MAPK gene family. Mol Biol Rep. DOI 10.1007/s11033-012-2184-9.

26. Zhao, P., Genhong Wang, Z. M. Dong, J. Duan, P. Z. Xu, T. C. Cheng, Z. H. Xiang and Q. Y. Xia (2010). "Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm Bombyx mori." BMC Genomics 11: 405.

27. Genhong Wang, Chun Liu, Xing-fu Zha, Jie Chen, Liang Jiang and Q.-y. Xia. (2008). "Cathepsin B protease is required for metamorphism in silkworm, Bombyx mori." Insect Science 15: 201-208.

28. Genhong Wang, Qing-You Xia, Dao-Jun Cheng, Jun Duan, Ping Zhao, Jie Chen and Z. Li. (2008). "Reference genes identified in the silkworm Bombyx mori during metamorphism based on oligonucleotide microarray and confirmed by qRT-PCR." Insect Science 15: 405-413.

29. The International Silkworm Genome, C. (2008) The genome of a lepidopteran model insect, the silkworm Bombyx mori. Insect Biochem Mol Biol, 38, 1036-45. (the 28th author in the Chinese group).

30. Cheng, D., Q. Xia, J. Duan, L. Wei, C. Huang, Z. Li, G. Wang and Z. Xiang (2008). "Nuclear receptors in Bombyx mori: Insights into genomic structure and developmental expression." Insect Biochem Mol Biol 38(12): 1130-7.

31. Qingyou Xia, Daojun Cheng, Jun Duan, Genhong Wang, Tingcai Cheng, Xingfu Zha, Chun Liu, Ping Zhao, Fangyin Dai, Ze Zhang, Ningjia He, Liang Zhang and Zhonghuai Xiang (2007). "Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori." Genome Biol 8(8): R162.

32. 王根洪, 祝慧敏, 罗会松, 王轲轲, 杨小波, 蒋亮, 夏庆友 (2011). "细菌表达dsRNA介导的家蚕FTZ-F1基因的RNA干扰." 昆虫学报 54(5): 596-601.

33. 王根洪, 刘春, 杨远萍, 吴雪峰, 夏庆友 (2005). "siRNA介导的家蚕ABC转运蛋白相关基因的干涉研究." 蚕业科学(2): 117-120,i001.

34. 艾均文, 王根洪, 李艳红, 余泉友, 张学松, 朱勇, 向仲怀. (2008). "家蚕P450基因CYP18A1的克隆、序列分析及其表达谱研究." 昆虫学报 3: 237-245.

35.王振兴, 王璞, 姚玲, 曾智东, 徐汉福, 段军, 王根洪, 夏庆友 (2006). "家蚕细胞凋亡相关基因BmlCAD的克隆、序列和功能分析及其在精巢中特异表达的初步研究." 遗传(7): 838-844.

36. 徐汉福, 王 峰, 王根洪, 段晓利, 马三垣, 夏庆友 (2011). "家蚕Poly(A)结合蛋白基因BmPABP的克隆及序列与功能分析." 蚕业科学 37(3): 412-418.

37. 杨远萍, 刘春, 王子龙, 王根洪, 夏庆友 (2006). "家蚕组织蛋白酶D基因的克隆、序列分析及其表达谱研究." 昆虫学报(2): 172-178.

联系地址：重庆市北碚区西南大学（南区）蚕学宫410室，400715

电    话： 023-68251573

E-mail：wanggh168@swu.edu.cn