张艳

副教授、硕士生导师

学习工作经历：

2003.09~2007.07   西南大学，学士

2007.09~2012.12   西南大学，博士（硕博连读）

2013.03~2015.03   西南大学，博士后

2015.04~2018.06   西南大学，助理研究员

2018.12-2019.12  英国华威大学，访问学者

2018.07~至今    西南大学，副教授

研究领域与方向：

1.昆虫的细胞自噬。自噬是一种进化上保守的分解代谢过程，其依赖溶酶体途径对胞

质蛋白和细胞器进行降解。自噬与衰老、神经退行性疾病、癌症、自身免疫病和心血管等疾病密切相关。我们主要以家蚕、果蝇等为实验对象，研究自噬在其中的作用，建立相关疾病模型，期望通过昆虫自噬的研究为人类疾病提供参考和借鉴。

2.昆虫的营养与代谢。昆虫是地球上数量最多、最为成功的一类动物。作为昆虫

中进化程度最高的完全变态昆虫一生要经历卵、幼虫、蛹和成虫四个阶段。无论是在卵期、蛹期或蜕皮变态期，昆虫均暂时停止进食，不能从外部获得营养。因此，昆虫的营养积累至关重要。课题组研究昆虫变态期的营养蛋白积累、储存、利用以及调控机理，为深入理解昆虫的发育变态奠定基础。

成果情况：

从事昆虫的营养与细胞自噬研究，已在Cell reports、Autophagy、STAR Protocols、Insect Biochem Mol Biol等杂志发表SCI文章50余篇；主持国家自然基金青年基金项目、重庆市自然科学基金面上项目、中国博士后科学基金项目等；与英国华威大学保持紧密合作关系。

代表性论文：

1. Yan Zhang*, Nezis IP*. Label-free quantitative proteomic analysis of adult Drosophila heads. STAR Protoc. 2022 Nov 11;3(4):101830.

2. Raksha Gohel, Ashrafur Rahman, Peter Lőrincz, Anikó Nagy, Gábor Csordás, Yan Zhang, Gábor Juhász, Ioannis P Nezis*. Selective autophagy and Golgi quality control in Drosophila. Autophagy. 2022 Oct;18(10):2508-2509.

3. Ashrafur Rahman, Peter Lőrincz, Raksha Gohel , Anikó Nagy, Gábor Csordás, Yan Zhang, Gábor Juhász, Ioannis P Nezis*. GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila. Cell Rep. 2022 May 31;39(9):110903.

4. Lin Ye, Yan Zhang, Zhaoming Dong, Pengchao Guo, Dongchao Zhao, Haoyun Li, Hang Hu,

Xiaofang Zhou, Haiqin Chen, Ping Zhao*. Five Silkworm 30K Proteins Are Involved in the Cellular

Immunity against Fungi. Insects 2021, 12, 107.

5. Yan Zhang, Muya Tang, Zhaoming Dong, Dongchao Zhao, Lingna An, Hongtao Zhu, Qingyou Xia, Ping Zhao*. Synthesis, secretion, and antifungal mechanism of a phosphatidylethanolamine-binding protein from the silk gland of the silkworm Bombyx mori. International Journal of Biological Macromolecules 149 (2020) 1000–1007.

6. Yan Zhang, Dongchao Zhao, Zhu Meng, Zhaoming Dong, Ying Lin, Shiyi Chen, Qingyou Xia,

and Ping Zhao. Wild Silkworm Cocoon Contains More Metabolites Than Domestic Silkworm Cocoon to Improve Its Protection. Journal of Insect Science, (2017) 17(5): 105; 1–6.

7. Yan Zhang, Ping Zhao, Zhaoming Dong, Dandan Wang, Xiaomeng Guo, Pengchao Guo, Qianru Song, Weiwei Zhang, Qingyou Xia*. Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori. PLoS One. 2015, 10(4): e0123403.

8. Yan Zhang, Zhaoming Dong, Peiming Gu, Weiwei Zhang, Dandan Wang,Xiaomeng Guo, Ping Zhao*, Qingyou Xia. Proteomics analysis of adult testis fromBombyx mori. Proteomics. 2014(14): 2345–2349.

9. Yan Zhang, Zhaoming Dong, Dandan Wang, Yong Wu, Qianru Song, Peiming Gu, Ping Zhao*, Qingyou Xia. Proteomics of larval hemolymph in Bombyx morireveals various nutrient-storage and immunity-related proteins. Amino Acids. 2014(46):1021-1031.

10. Yan Zhang, Ping Zhao, Hongli Liu, Zhaoming Dong, Qiang Yang, Dandan Wang, Qingyou Xia*. The synthesis, transportation and degradation of BmLP3 and BmLP7, two highly homologous Bombyx mori 30K proteins. Insect biochemistry and molecular biology. 2012(42): 827-834.

11. Yan Zhang, Zhaoming Dong, Shiping Liu, Qiang Yang, Ping Zhao, Qingyou Xia*. Identification of novel members reveals the structural and functional divergence of lepidopteran-specific Lipoprotein_11 family. Functional & Integrative Genomics. 2012(12): 705-715.

12. 张艳，董照明，赵东超，李浩云，王凌燕，林英，赵萍. 基于超高效液相色谱-质谱技术对野蚕茧和家蚕茧化学成分进行比较.生物工程学报, 2019,35(8): 1546–1556.

13. 张艳，董照明，席星航，张晓璐，叶林，郭凯雨，夏庆友，赵萍. 家蚕脱胶蚕丝的蛋白组成成分. 中国农业科学 2018,51(11):2216-2224.

14. Zhaoming Dong, Kaiyu Guo, Xiaolu Zhang, Tong Zhang, Yan Zhang, SanyuanMa, Huaipu Chang, Muya Tang, Lingna An, Qingyou Xia, Ping Zhao. Identification of Bombyx mori sericin 4 protein as a new biological adhesive. International Journal of Biological Macromolecules 2019 (132) 1121–1130.

15. Muya Tang, Zhaoming Dong, Pengchao Guo, Yan Zhang, Xiaolu Zhang, Kaiyu Guo, Lingna An, Xinyang Liu, Ping Zhao. Functional analysis and characterization of antimicrobial phosphatidylethanolamine-binding protein BmPEBP in the silkworm Bombyx mori. Insect Biochemistry and Molecular Biology 110 (2019) 1–9.

16. Zhongyan Lu, Zhu Meng, Maoyu Wen, Xiaoli Kang, Yan Zhang, Qingsong Liu, Ping Zhao, Qingyou Xia, Overexpression of BmFoxO inhibited larval growth and promoted

glucose synthesis and lipolysis in silkworm. Molecular Genetics and Genomics (2019) 294:1375–1383.

17. Shiyi Chen, Zhaoming Dong, Xiu Ren, Dongchao Zhao, Yan Zhang, Muya Tang, Jiaxuan Han, Lin Ye, Ping Zhao. Proteomic Identification of Immune-Related Silkworm Proteins Involved in the Response to Bacterial Infection. Journal of Insect Science, (2019) 19(4): 13; 1–11.

18. Zhaoming Dong, Lin Ye, Yan Zhang, Zhiyong Chen, Benchi Li,Tao Zhang,1,2,3 and Ping Zhao. Identification of N-linked Glycoproteins in Silkworm Serum Using Con A Lectin Affinity Chromatography and Mass Spectrometry. Journal of Insect Science, (2021) 21(4): 14; 1–11.

19. Hong-Lei Li, Lin Wu, Zhaoming Dong, Yusong Jiang, Sanjie Jiang, Haitao Xing, Qiang Li, Guocheng Liu, Shuming Tian, Zhangyan Wu, Bin Wu, Zhexin Li, Ping Zhao, Yan Zhang, Jianmin Tang, Jiabao Xu, Ke Huang, Xia Liu, Wenlin Zhang, Qinhong Liao, Yun Ren, Xinzheng Huang, Qingzhi Li, Chengyong Li, Yi Wang, Baskaran Xavier-Ravi, Honghai Li, Yang Liu, Tao Wan, Qinhu Liu, Yong Zou , Jianbo Jian, Qingyou Xia and Yiqing Liu. Haplotype-resolved genome of diploid

ginger (Zingiber officinale) and its unique gingerol biosynthetic pathway. Hortic. Res. 8, 189.

20. Zhaoming Dong, Lingna An, Mengyao Lu, Muya Tang, Haiqin Chen, Xuan Huang, Yong Hou, Guanwang Shen, Xiaolu Zhang, Yan Zhang, Qingyou Xia, Ping Zhao. SPINK7 Recognizes Fungi and Initiates Hemocyte-Mediated Immune Defense Against Fungal Infections. Front. Immunol. 12:735497.

21. Yutian Lei, Kaiyu Guo, Yan Zhang, Xiaolu Zhang , Lixia Qin , Xin Wang, Hongtao Zhu , Yuanyuan Guo, Wenxin Yang , Benchi Li , Qingyou Xia, Ping Zhao, Zhaoming Dong.  Adhesive property and mechanism of silkworm egg glue protein.  Acta Biomaterialia 134 (2021) 499–512.

22. Xiaoli Kang, Yaping Wang, Wenjuan Liang, Xin Tang, Yan Zhang, Lingyan Wang, Ping Zhao, Zhongyan Lu. Bombyx mori nucleopolyhedrovirus downregulates transcription factor BmFoxO to elevate virus infection. Dev Comp Immunol. 2021 Mar;116:103904.

23. Kaiyu Guo, Xiaolu Zhang, Zhaoming Dong, Yuhui Ni, Yuqing Chen, Yan Zhang, Haoyun Li, Qingyou Xia, Ping Zhao. Ultrafine and High-Strength Silk Fibers Secreted by Bimolter Silkworms. Polymers 2020, 12, 2537; doi:10.3390/polym12112537.

24. Xiaolu Zhang, Kaiyu Guo, Zhaoming Dong, Zhiyong Chen, Hongtao Zhu,Yan Zhang, Qingyou Xia, Ping Zhao. Kunitz-type protease inhibitor BmSPI51 plays an antifungal role in the silkworm cocoon. Insect Biochemistry and Molecular Biology 116 (2020) 103258.

25. Hongtao Zhu, Xiaolu Zhang, Mengyao Lu, Haiqin Chen, Shiyi Chen, Jiaxuan Han, Yan Zhang,  Ping Zhao, Zhaoming Dong. Antibacterial Mechanism of Silkworm Seroins. Polymers 2020, 12, 2985.

26. Lingyan Wang, Yaru Yin, Kun Wang, Jun Cao, Tingcai Cheng, Chun Liu, Yan Zhang, Yong Zhu.  Bombyx mori monocarboxylate transporter 9 (BmMCT9) is involved in the transport of uric acid in silkworm integument. Genes Cells 2020 Jan;25(1):33-40.

27. Zhaoming Dong, Kaiyu Guo, Xiaolu Zhang, Tong Zhang, Yan Zhang, SanyuanMa,

Huaipu Chang, Muya Tang, Lingna An, Qingyou Xia, Ping Zhao. Identification of Bombyx mori sericin 4 protein as a new biological adhesive. International Journal of Biological Macromolecules 2019 (132) 1121–1130.

28. Muya Tang, Zhaoming Dong, Pengchao Guo, Yan Zhang, Xiaolu Zhang, Kaiyu Guo, Lingna An, Xinyang Liu, Ping Zhao. Functional analysis and characterization of antimicrobial phosphatidylethanolamine-binding protein BmPEBP in the silkworm Bombyx mori. Insect Biochemistry and Molecular Biology 110 (2019) 1–9.

29. Zhongyan Lu, Zhu Meng, Maoyu Wen, Xiaoli Kang, Yan Zhang, Qingsong Liu, Ping Zhao, Qingyou Xia, Overexpression of BmFoxO inhibited larval growth and promoted

glucose synthesis and lipolysis in silkworm. Molecular Genetics and Genomics (2019) 294:1375–1383.

30. Lixia Qin, Jing Li, Kaiyu Guo, Mengyao Lu, Yan Zhang, Xiaolu Zhang, Yanqiong Zeng, Xin Wang, Qingyou Xia, Ping Zhao, Ai-Bing Zhang, Zhaoming Dong*. Insights into the structure and composition of mineralized hard cocoons constructed by the oriental moth, Monema (Cnidocampa) flavescens Walker. Insect Biochem Mol Biol. 2022 Dec;151:103878.

31. Shiyi Chen, Zhaoming Dong, Xiu Ren, Dongchao Zhao, Yan Zhang, Muya Tang, Jiaxuan Han, Lin Ye, Ping Zhao. Proteomic Identification of Immune-Related Silkworm Proteins Involved in the Response to Bacterial Infection. Journal of Insect Science, (2019) 19(4): 13; 1–11.

32.  Kaiyu Guo, Zhaoming Dong, Yan Zhang, Dandan Wang, Muya Tang, Xiaolu Zhang,

Qingyou Xia, Ping Zhao. Improved strength of silk fibers in Bombyx mori trimolters induced by an anti-juvenile hormone compound. BBA - General Subjects 1862 (2018) 1148–1156.

33. Xiaolu Zhang, Huaipu Chang, Zhaoming Dong, Yan Zhang, Dongchao Zhao, Lin Ye,

Qingyou Xia, and Ping Zhao. Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin-Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage. Proteomics 2018, 18, 1700389.

34. Dandan Wang, Zhaoming Dong, Yan Zhang, Kaiyu Guo, Pengchao Guo, Ping Zhao, and Qingyou Xia. Proteomics Provides Insight into the Interaction between Mulberry and Silkworm. J. Proteome Res. 2017, 16, 2472−2480.

35. Dandan Wang, Yan Zhang, Zhaoming Dong, Pengchao Guo, Sanyuan Ma, Kaiyu Guo, Qingyou Xia, Ping Zhao.Serine protease P-IIc is responsible for the digestion of yolk proteins at the late stage of silkworm embryogenesis. Insect Biochem Mol Biol. 2016, 74:42-49.

36. Yong Hou, Yan Zhang, Jing Gong, Sha Tian, Jianwei Li, Zhaoming Dong, Chao Guo, Li Peng, Ping Zhao, Qingyou Xia. Comparative proteomics analysis of silkworm hemolymph during the stages of metamorphosis via liquid chromatography and mass spectrometry. Proteomics. 2016,16(9):1421-1431.

37. Zhaoming Dong, Qianru Song, Yan Zhang, Shiyi Chen, Xiaolu Zhang, Ping Zhao, Qingyou Xia. Structure, evolution, and expression of antimicrobial silk proteins, seroins in Lepidoptera. Insect biochemistry and molecular biology. 2016, 75: 24-31.

38. Zhaoming Dong, Ping Zhao, Yan Zhang, Qianru Song, Xiaolu Zhang, Pengchao Guo, Dandan Wang, Qingyou Xia. Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori. Scientific Reports. 2016, 6: 21158.

39. Zhaoming Dong, Weiwei Zhang, Yan Zhang, Xiaolu Zhang, Ping Zhao, Qingyou Xia. Identification and characterization of novel chitin-binding proteins from the larval cuticle of silkworm, Bombyx mori. Journal of Proteome Research. 2016, 15(5): 1435-1445.

40. Zhaoming Dong, Xiaohuan Wang, Yan Zhang, Liping Zhang, Quanmei Chen, Xiaolu Zhang, Ping Zhao, Qingyou Xia. Proteome profiling reveals tissue-specific protein expression in male and female accessory glands of the silkworm, Bombyx mori. Amino Acids. 2016, 48(5): 1173-1183.

41. Xiaomeng Guo, Zhaoming Dong, Yan Zhang, Youshan Li, Huawei Liu, Qingyou Xia, Ping Zhao. Proteins in the Cocoon of Silkworm Inhibit the Growth of Beauveria bassiana. PLoS One. 2016, 11(3):e0151764.

42. Liu L, Wang Y, Li Y, Lin Y, Hou Y, Zhang Y, Wei S, Zhao P, Zhao P, He H. LBD1 of Vitellogenin Receptor Specifically Binds to the Female-Specific Storage Protein SP1 via LBR1 and LBR3. PLoS One. 2016,11(9):e0162317.

43. Yi Li, Xin Wang, Yong Hou, Xiaoying Zhou, Quanmei Chen, Chao Guo, Qingyou Xia, Yan Zhang, Ping Zhao. Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior. J Proteome Res. 2016, 15(1):193-204.

44. Peng-Chao Guo', Zhaoming Dong', Ping Zhao, Yan Zhang, Huawei He, Xiang Tan, Weiwei Zhang, Qingyou Xia. Structural insights into the unique inhibitory mechanism of the silkworm protease inhibitor serpin18. Scientific Reports, 2015, 5: 11863.

45. Pengchao Guo, Zhaoming Dong, Li Xiao, Tao Li, Yan Zhang, Huawei He, Qingyou Xia, Ping Zhao. Silk gland-specific proteinase inhibitor serpin16 from the Bombyx mori shows cysteine proteinase inhibitory activity. Biochemical and Biophysical Research Communications. 2015, 457(1): 31–36.

46. Quanmei Chen, Zhengang Ma, Xin Wang, Zhiqing L, Yan Zhang, Sanyuan Ma, Ping Zhao, Qingyou Xia. Comparative proteomic analysis of silkworm fat body after knocking out fibroin heavy chain gene: a novel insight into cross-talk between tissues. Funct Integr Genomics. 2015(5):611-37.

47. Chen Z, Nohata J, Guo H, Li S, Liu J, Guo Y, Yamamoto K, Kadono-Okuda K, Liu C, Arunkumar KP, Nagaraju J, Zhang Y, Liu S, Labropoulou V, Swevers L, Tsitoura P, Iatrou K, Gopinathan KP, Goldsmith MR, Xia Q, Mita K. A comprehensive analysis of the chorion locus in silkmoth. Scientific Reports. 2015, 5:16424.

48. Zhaoming Dong, Ping Zhao, Chen Wang, Yan Zhang, Jianping Chen, Xin Wang, Ying Lin, Qingyou Xia*. Comparative Proteomics Reveal Diverse Functions and Dynamic Changes of Bombyx Mori Silk Proteins Spun From Different Development Stages. Journal of Proteome Research. 2013, 11 (12): 5213-5222.

49. 董照明，张艳，杨佩，赵萍，席星航，夏庆友. 蚕丝的基础研究与应用：从纤维到蛋白质的转型. 蚕业科学, 2015, 41 (3): 395-404.

50. 赵萍，张艳，钟晓武，王凌燕，谭祥，董照明，莫现会，夏庆友. 胰凝乳蛋白酶抑制剂在蓖麻蚕主要组织中的分布及血液中的含量和活性. 昆虫学报, 2016, 59(2) : 138-147.

51. 王凌燕，钟晓武，张艳，董照明，王娟，夏庆友，赵萍.不同家蚕品系的胰蛋白酶抑制剂种类与活性的多态性分析. 蚕业科学, 2016, 42(04): 613-618.

52. 陈建平，赵萍*，董照明，张艳，石虎，夏庆友.参与家蚕免疫反应的 clip 丝氨酸蛋白酶家族基因分析. 蚕业科学, 2012, 38(2): 0241-0248.

53. 张薇薇，董照明，张艳，张晓璐，张守亚，赵萍. 家蚕表皮蛋白BmCPAP3-G的表达特征及其与几丁质的结合特性. 中国农业科学  2017,50(9):1723-1733.

联系方式：

Email：zhangy66@swu.edu.cn