研究队伍
副高
当前位置: 首页 > 研究队伍 > 科研队伍 > 正文
王 鑫
发布时间: 2022年04月11日 10:55   作者:   审核人:  来源:   浏览次数:

   

副教授、硕士生导师

2020.7至今

西南大学前沿交叉学科研究院生物学研究中心

副教授

2018.8 – 2020.6

西南大学家蚕基因组生物学国家重点实验室

助理研究员

2016.7 – 2018.7

西南大学家蚕基因组生物学国家重点实验室

师资博士后

2010.9 – 2016.6

西南大学家蚕基因组生物学国家重点实验室

博士(硕博连读)

2006.9 – 2010.7

成都理工大学

学士


主要科研领域与方向:

1.蚕丝纤维成纤机制的研究:利用多组学的手段鉴定家蚕丝腺中与蚕丝成纤维相关的因子,系统性研究成纤维因子在蚕丝性能决定中的作用,阐释蚕丝蛋白成纤维的分子机制。

2.高性能新型蚕丝纤维的开发和利用:通过遗传操作手段改变家蚕成纤维因子,以金属离子、表皮蛋白等为靶标,对蚕丝纤维的性能进行定向改良,创制新型高性能蚕丝纤维品种与素材。

科学成就:

1.蚕丝成纤维机制研究理论有创新:首次提出丝腺中纤维化过程决定蚕丝性能的理论,鉴定了关键的成纤维因子,并对其功能进行了深入研究,从分子层面解析了蚕丝蛋白纤维化的机理。

2.新型高性能纤维拓展应用有突破:通过对蚕丝成纤维因子的遗传改造,成功创制多款力学性能显著提升的新型蚕丝纤维,其中“铁蚕丝”纤维较对照提升1.5倍,刚度和韧度提升2倍以上,生丝纤维强伸度指标远超国标水平,具有重大应用价值,正进行推广。

3.基于获得的系列原创性、开拓性成果,发表研究论文30余篇,其中以第一作者或通讯作者在Acta Biomaterialia、Biomacromolecules、Insect Biochemistry and Molecular Biology、Biochimica et Biophysica Acta、Proteomics等权威杂志发表论文11篇;获国家发明专利授权4项;主持国家自然科学基金面上项目,国家自然科学基金青年基金项目、中国博士后科学基金一等资助、重庆市博士后研究项目特别资助项目,并参加了国家973等重大课题的研究。担任Biomacromolecules、International Journal of Biological Macromolecules等SCI期刊的审稿人。

指导学生:

1.讲授课程:本科课程:《生物化学》、《生物艺术赏析》;

2.研究生课程:《分子生物学实验原理与技术》、《生物化学大实验》。

3.指导学生项目:蚕丝提铀技术研发,西南大学大学生创新创业训练计划项目,2023.5-2024.5

4.指导学生获奖:2022年全国大学生生物化学歌曲大赛全国二等奖

主持科研项目:

1.国家自然科学基金面上项目,家蚕前部丝腺特异表皮蛋白在角质层内膜构建及蚕丝纤维化中的功能研究,2023/1-2026/12

2.中央高校基本科研业务费,蚕丝性能决定因子在高性能蚕丝素材创制中的应用,2022/1-2024/12

3.国家自然科学基金青年基金项目,家蚕纺丝管道与蚕丝力学性能定向改良,2020/1-2022/12

4.西南大学博士启动基金项目,金属离子介导蚕丝力学性能定向改良的基础研究,2018/9-2020/12

5.中国博士后科学基金第60批面上项目一等资助,蚕丝纤维化机制研究及新型蚕丝纤维的开发,2016/7-2018/6

6.重庆市2017年博士后科研项目特别资助二等资助,利用金属离子对蚕丝纤维性能进行定向改良的基础研究,2017/7-2018/6

 代表性论文/论著:

1.Wang X#, Xie X#, Xie K, Liu Q, Li Y, Tan X, Dong H, Li X, Dong Z, Xia Q, Zhao P*, Chitin and cuticle proteins form the cuticular layer in the spinning duct of silkworm, Acta Biomater, 2022, 145: 260-271. Link: https://doi.org/10.1016/j.actbio.2022.03.043

2.Wang X#, Zhao P#, Li Y, Yi Q, Ma S, Xie K, Chen H, Xia Q*, Modifying the mechanical properties of silk fiber by genetically disrupting the ionic environment for silk formation, Biomacromolecules, 2015, 16(10): 3119-3125. Link: https://doi.org/10.1021/acs.biomac.5b00724

3.Wang X, Li Y, Liu Q, Tan X, Xie X, Xia Q, Zhao P*, GC/MS-based metabolomics analysis reveals active fatty acids biosynthesis in the Filippi's gland of the silkworm, Bombyx mori, during silk spinning, Insect Biochem Mol Biol, 2019, 105: 1-9. Link: https://doi.org/10.1016/j.ibmb.2018.12.009

4.Wang X, Li Y, Peng L, Chen H, Xia Q, Zhao P*, Comparative transcriptome analysis of Bombyx mori spinnerets and Filippi's glands suggests their role in silk fiber formation, Insect Biochem Mol Biol, 2016, 68: 89-99. Link: https://doi.org/10.1016/j.ibmb.2015.11.003

5.Wang X#, Tan X#, Liu Q, Li Y, Li X, Dong Z, Dong H, Xia Q, Zhao P*, Fiber formation and mechanical properties of Bombyx mori silk are regulated by vacuolar-type ATPase, ACS Biomater Sci Eng, 2021, 7(12): 5532–5540. Link: https://doi.org/10.1021/acsbiomaterials.1c01230

6.Wang X, Li Y, Peng L, Chen H, Xia Q, Zhao P*, In vivo effects of metal ions on conformation and mechanical performance of silkworm silks, Biochim Biophys Acta, 2017, 1861(3): 567-576. Link: https://doi.org/10.1016/j.bbagen.2016.11.025

7.Wang X, Li Y, Liu Q, Xia Q, Zhao P*, Proteome profile of spinneret from the silkworm, Bombyx mori, Proteomics, 2017, 17(12): 1600301. Link: https://doi.org/10.1002/pmic.201600301

8.Wang X, Li Y, Xie K, Yi Q, Chen Q, Wang X, Shen H, Xia Q, Zhao P*, Ca2+ and endoplasmic reticulum Ca2+-ATPase regulate the formation of silk fibers with favorable mechanical properties, J Insect Physiol, 2015, 73: 53-59. Link: https://doi.org/10.1016/j.jinsphys.2015.01.002

9.王鑫, 李懿, 陈全梅, 衣启营, 谢康, 吴用, 赵萍*, 家蚕空泡型ATP酶(V-ATPase)基因的基本信息及表达特征, 中国农业科学, 2014, 47(8): 1611-1621.

10.王鑫, 李懿, 陈慧芳, 周小英, 谢康, 赵萍*, 利用家蚕杆状病毒表达家蚕肌质网型钙离子ATP酶蛋白, 生物工程学报, 2015, 31(3): 421-430.

11.王鑫, 李懿, 刘青松, 李心宁, 夏庆友, 赵萍*, 硝酸钙盐缩处理对生丝结构与力学性能的影响, 西南大学学报(自然科学版), 2022, 44(8): 77-84.

12.Xie X, Wang X, Liu Q, Li Y, Dong Z, Wang L, Xia Q, Zhao P*, The tissue-specific expression of silkworm cuticle protein gene ASSCP2 is mediated by the Sox-2 transcription factor, Int J Biol Macromol, 2023, 237: 124182.

13.Li Y, Wang X, Xie X, Liu Q, Dong H, Hou Y, Xia Q, Zhao P*, Enhanced locomotor behaviour is mediated by activation of tyrosine hydroxylase in the silkworm brain, Insect Mol Biol, 2023, 32(3): 251-262.

14.Li Y, Wang X, Dong H, Xia Q, Zhao P*, Transcriptomic analysis of starvation on the silkworm brain, Insects, 2023, 14(7): 658.

15.Li Y, Wang X, Hou Y, Zhou X, Chen Q, Guo C, Xia Q, Zhang Y, Zhao P*, Integrative proteomics and metabolomics analysis of insect larva brain: Novel insights into the molecular mechanism of insect wandering behavior, J Proteome Res, 2016, 15(1): 193-204.

16.Li Y, Wang X, Chen Q, Hou Y, Xia Q, Zhao P*, Metabolomics Analysis of the Larval Head of the Silkworm, Bombyx mori, Int J Mol Sci, 2016,17(9): 1460.

17.Liu Q, Wang X, Tan X, Xie X, Li Y, Zhao P, Xia Q*, A strategy for improving the mechanical properties of silk fiber by directly injection of ferric ions into silkworm, Mater Des, 2018, 146: 134-141.

18.Liu Q, Wang X, Tan X, Xie X, Dong H,  Li X. Li Y, Zhao P, Xia Q*, Disruption of the metal ion environment by EDTA for silk formation affects the mechanical properties of silkworm silk, Int J Mol Sci, 2019, 20: 3026.

19.Liu Q, Wang X, Zhou Y, Tan X, Xie X, Li Y, Dong Z, Tang Z, Zhao P, Xia Q*, Dynamic changes and characterization of the metal ions in the silk glands and silk fibers of silkworm, Int J Mol Sci, 2023, 24(7): 6556.

20.Tan X, Wang X, Liu Q, Xie X, Li Y, Li B, Li Z, Xia Q, Zhao P*, Inhibition of silkworm vacuolar-type ATPase activity by its inhibitor Bafilomycin A1 induces caspase-dependent apoptosis in an embryonic cell line of silkworm, Arch Insect Biochem Physiol, 2018, 99(3): e21507.

21.Yi Q, Zhao P, Wang X, Zou Y, Zhong X, Wang C, Xiang Z, Xia Q*, Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process, Proteomics, 2013, 13(17): 2657-2663.

22.Chen Q, Ma Z, Wang X, Li Z, Zhang Y, Ma S, Zhao P*, Xia Q, Comparative proteomic analysis of silkworm fat body after knocking out fibroin heavy chain gene: a novel insight into cross-talk between tissues, Funct Integr Genomics, 2015, 15(5): 611-637.

23.Cai R, Chen X, Yang W, Wang X, Sun L, Zhao P, Xia Q, He H*, Wang Y*, POUM2 homeostasis regulates intimal remodeling and cells fate in the anterior silk gland of the silkworm, Int J Biol Macromol, 2023, 225: 715-729.

24.Tang X, Liu H, Chen Q, Wang X, Xiong Y, Zhao P*, Genome-wide identification, characterization and expression analysis of the solute carrier 6 gene family in silkworm (Bombyx mori), Int J Mol Sci, 2016, 17(10): 1675.

25.Iqbal MI, Sun F, Fei B, Xia Q, Wang X, Hu J*. Knit architecture for water-actuating woolen knitwear and its personalized thermal management, ACS Appl Mater Interfaces, 2021, 13(5): 6298-6308.

26.Shen H, Yan W, Yang X*, He X, Wang X, Zhang Y, Wang B, Xia Q*, Co-occurrence network analyses of rhizosphere soil microbial PLFAs and metabolites over continuous cropping seasons in tobacco, Plant Soil, 2020, 452: 119-135.

27.Peng Z, Yang X, Liu C, Dong Z, Wang F, Wang X, Hu W, Zhang X, Zhao P, Xia Q*, Structural and mechanical properties of silk from different instars of Bombyx mori, Biomacromolecules, 2019, 20(3):1203-1216.

28.Lei Y, Guo K, Zhang Y, Zhang X, Qin L, Wang X, Zhu H, Guo Y, Yang W, Li B, Xia Q, Zhao P*, Dong Z*. Adhesive property and mechanism of silkworm egg glue protein, Acta Biomater. 2021, 134: 499-512.

29.Dong Z, Zhao P, Wang C, Zhang Y, Chen J, Wang X, Lin Y, Xia Q*, Comparative proteomics reveal diverse functions and dynamic changes of Bombyx mori silk proteins spun from different development stages, J Proteome Res, 2013, 12(11): 5213-5222.

30.Qin L, Li J, Guo K, Lu M, Zhang Y, Zhang X, Zeng Y, Wang X, Xia Q, Zhao P, Zhang AB, Dong Z*, Insights into the structure and composition of mineralized hard cocoons constructed by the oriental moth, Monema (Cnidocampa) flavescens Walker. Insect Biochem Mol Biol, 2023,151: 103878.

31.谢康, 王鑫, 陈慧芳, 李懿, 宋倩茹, 赵萍*, 家蚕前部丝腺特异表皮蛋白Bm11721的鉴定及表达, 生物工程学报, 2016, 32(1): 64-73.

32.陈慧芳, 王鑫, 谢康, 李懿, 赵萍*, 家蚕V型ATP酶B亚基的克隆及表达特征, 生物工程学报, 2016, 32(4): 487-496.

33.陈全梅, 马振刚, 王晓欢, 孙艳慧, 王鑫, 代云香, 赵萍*, 家蚕感受神经膜蛋白基因SNMP1的序列分析及原核表达, 蚕业科学, 2013, 39(2): 231-239.

34.马三垣, 王鑫, 胡文波, 夏庆友, 转基因技术让蚕宝宝吐蜘蛛丝, 人与生物圈, 2018, 6: 50-53.


联系地址:重庆市北碚区西南大学蚕学宫411,400715

E-mail:swuwangxin@swu.edu.cn

欢迎有兴趣从事以上研究的同学报考研究生,欢迎本科同学加入课题组进行科研训练。