郭凯雨

副教授，硕士生导师

学习工作经历

2010.09-2015.06，西北农林科技大学动物医学，农学学士

2015.09-2021.06，西南大学生物化学与分子生物学，理学博士

2021.06-2024.01， 西南大学畜牧学，博士后

2024.03至今 西南大学 副教授

研究领域

1. 昆虫丝纤维组分的鉴定及功能研究。利用多组学手段分析昆虫丝中的高丰度蛋白，系统性研究不同丝蛋白的生物学功能。

2. 基于CRISPR/Cas9基因编辑和转基因技术创制新型蚕丝素材。通过遗传操作手段改变蚕丝纤维蛋白成分，开发多功能蚕丝蛋白材料。

3. 开发丝蛋白材料用于小口径丝素血管移植物的制备，探索丝蛋白在干细胞培养和干细胞治疗领域的应用。

成果情况

发明专利

1. 赵萍; 郭凯雨; 张晓璐; 夏庆友; 具有抗菌活性的Sericin3蛋白的重复基序及其应用, 中国，ZL 202211030502；

2. 赵萍; 郭凯雨; 张晓璐; 董照明；夏庆友; 家蚕丝胶蛋白Ser4在提高蚕丝性能中的应用及其方法，中国，ZL 202211572164；

3. 赵萍; 张晓璐; 郭凯雨; 董照明; 夏庆友; 家蚕BmSPI51突变蛋白BmSPI51M及其制备方法和应用, 中国, ZL 2021101741434；

4. 赵萍; 张晓璐; 董照明;郭凯雨;夏庆友; 敲低Seroin1基因的家蚕双元转基因系统及家蚕纯裸蛹品种的制备方法，中国，ZL 2020109411048。

承担项目

1、国家自然科学基金青年项目：家蚕小蚕期丝胶蛋白在丝纤维形成中的功能及其生物学特性研究，起止时间：202401-202612，主持；

2、重庆市博士后面上资助项目：家蚕丝胶层蛋白P25-like在丝纤维中的功能研究，202208-202403，主持；

3、重庆市博士生科研创新项目：家蚕新丝胶蛋白的功能研究，201907-202105，主持；

4、国家自然科学基金面上项目：家蚕新丝胶蛋白的鉴定与功能研究，起止时间：202201-202512，主研。

代表性论文

1. Guo K, Dong Z, Zhang X, Chen Y, Li Y, Jiang W, Qin L, Liu X, Zhang Y, Guo Z, Xia Q, Zhao P. Analysis of histomorphometric and proteome dynamics inside the silk gland lumen of Bombyx mori revealed the dynamic change of silk protein during the molt stage. International Journal of Biological Macromolecules, 2023, 236: 123926.

2. Guo K, Zhang X, Zhao D, Qin L, Jiang W, Hu Wen, Liu X, Xia Q, Dong Z, Zhao P. Identification and characterization of sericin5 reveals non-cocoon silk sericin components with high β-sheet content and adhesive strength. Acta Biomaterialia, 2022, 150, 96–110.

3. Guo K, Dong Z, Zhang Y, Wang D, Tang M, Zhang X, Xia Q, Zhao P. Improved strength of silk fibers in Bombyx mori trimolters induced by an anti-juvenile hormone compound. BBA - General Subjects, 2018, (5) 1148-1156.

4. Guo K, Zhang X, Dong Z, Ni Y, Chen Y, Zhang Y, Li H, Xia Q, Zhao P. Ultrafine and high-strength silk fibers secreted by bimolter silkworms. Polymers, 12(11) (2020) e2537.

5. Dong Z#, Guo K#, Zhang X, Zhang T, Zhang Y, Ma S, Chang H, Tang M, An L, Xia, Q, Zhao P. Identification of Bombyx mori sericin 4 protein as a new biological adhesive. International Journal of Biological Macromolecules, 2019, 132: 1121-1130. (Co-first author).

6. Zhang X#, Guo K#, Dong Z, Chen Z, Zhu H, Zhang Y, Xia Q, Zhao P. Kunitz-type protease inhibitor BmSPI51 plays an antifungal role in the silkworm cocoon. Insect Biochem Mol Biol, 2020, 116: 103258. (Co-first author)

7. Jiang W#, Guo K#, Dong H, Zhang X, Guo Z, Duan J, Jing X, Xia Q, Zhao P. Mutation in the Bombyx mori BmGMC2 gene impacts silk production and silk protein synthesis. International Journal of Biological Macromolecules, 2024, 274: 133400. (Co-first author)

8. Lei Y, Guo K, Zhang Y, Zhang X, Qin L, Wang X, Zhu H, Guo Y, Yang W, Li B, Xia Q, Zhao P, Dong Z, Adhesive property and mechanism of silkworm egg glue protein, Acta Biomaterialia, 134 (2021) 499-512.

9. Qin L, Li J, Guo K, Lu M, Zhang Y, Zhang X, Zeng Y, Wang X, Xia Q, Zhao P, Zhang A, Dong Z, Insights into the structure and composition of mineralized hard cocoons constructed by the oriental moth, Monema (Cnidocampa) flavescens Walker, Insect Biochem Mol Biol, 2022, 151:103878.

10. Zhang X, Dong Z, Guo K, Jiang W, Wu X, Duan J, Jing X, Xia Q, Zhao P, Identification and functional study of fhx-L1, a major silk component in Bombyx mori, International Journal of Biological Macromolecules, 2023, 232: 123371.

11. Zhang X, Ni Y, Guo K, Dong Z, Chen Y, Zhu H, Xia Q, Zhao P. The mutation of SPI51, a protease inhibitor of silkworm, resulted in the change of antifungal activity during domestication. International Journal of Biological Macromolecules, 2021, 178: 63-70.

12. Wang W, Zhang F, Guo K, Xu J, Zhao P, Xia Q. CRISPR/Cas9-mediated gene editing of the let-7 seed sequence improves silk yield in the silkworm, Bombyx mori. International Journal of Biological Macromolecules, 2023, 243, 124793.

13.Wang D, Dong Z, Zhang Y, Guo K, Guo P, Zhao P, Xia Q. Proteomics provides insight into the interaction between mulberry and silkworm. J.Proteome Res. 2017, 16, 2472–2480.

14. Tang M, Dong Z, Guo P, Zhang Y, Zhang X, Guo K, An L, Liu X, Zhao P. Functional analysis and characterization of antimicrobial phosphatidylethanolamine-binding protein BmPEBP in the silkworm Bombyx mori. Insect Biochem Mol Biol, 2019, 110:1-9.

15. Wang D, Zhang Y, Dong Z, Guo P, Ma S, Guo K, Zhao P, Serine protease P-IIc is responsible for the digestion of yolk proteins at the late stage of silkworm embryogenesis[J]. Insect Biochem Mol Biol, 2016, 74:42-49.

16. 张艳，董照明，席星航，张晓璐，叶林，郭凯雨，夏庆友，赵萍. 家蚕脱胶蚕丝的蛋白组成成分[J].中国农业科学, 2018,51 (11):2216-2224.

联系方式

Email：gky2021@swu.edu.cn

微信号：guokaiyu1