孙乐

博士，博士后

2011-2015，石河子大学生物技术，理学学士

2015-2025，西南大学生物化学与分子生物学，理学博士

2025至今， 西南大学生物学，博士后

研究领域

1.家蚕丝蛋白基因饱和编辑

2.家蚕基因编辑工具扩展与开发

3.家蚕全基因组敲除文库筛选

成果情况

代表性论文/论著

1.Sun L, Wang R, Ma S, Shi R, Zhao P, Xia Q. Complete BmFib-L knockout reveals its indispensable role in silk fiber formation. Int J Biol Macromol. 2025 Mar; 294:138759.

2.Sun L, Zhang T, Lan X, Zhang N, Wang R, Ma S, Zhao P, Xia Q. High-throughput screening of PAM-Flexible Cas9 variants for expanded genome editing in the silkworm (Bombyx mori). Insects. 2024 Mar 30;15(4):241.

3.Lu W#, Ma S#, Sun L#, Zhang T, Wang X, Feng M, Wang A, Shi R, Jia L, Xia Q. Combined CRISPR toolkits reveal the domestication landscape and function of the ultra-long and highly repetitive silk genes. Acta Biomater. 2023 Mar 1;158: 190-202.

4.Ma S, Zhang T, Wang R, Wang P, Liu Y, Chang J, Wang A, Lan X, Sun L, Sun H, Shi R, Lu W, Liu D, Zhang N, Hu W, Wang X, Xing W, Jia L, Xia Q. High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism, Bombyx mori. Genome Res. 2024 Feb 7;34(1):134-144.

5.Cai R, Chen X, Yang W, Wang X, Sun L, Zhao P, Xia Q, He H, Wang Y. POUM2 homeostasis regulates intimal remodeling and cells fate in the anterior silk gland of the silkworm. Int J Biol Macromol. 2023 Jan 15; 225:715-729.

6.Liu Y, Yang C, Sun L, Wang A, Lan X, Xu W, Liang Y, Ma S, Xia Q. In-depth transcriptome unveils the cadmium toxicology and a novel metallothionein in silkworm. Chemosphere. 2021 Jun; 273:128522.

7.Wang X, Ma S, Liu Y, Lu W, Sun L, Zhao P, Xia Q. Transcriptional repression of endogenous genes in BmE cells using CRISPRi system. Insect Biochem Mol Biol. 2019 Aug;111:103172.

8.Ma S, Xia X, Li Y, Sun L, Liu Y, Liu Y, Wang X, Shi R, Chang J, Zhao P, Xia Q. Increasing the yield of middle silk gland expression system through transgenic knock-down of endogenous sericin-1. Mol Genet Genomics. 2017 Aug;292(4):823-831.